A. K2 accretion corrections1) Navigate to an abandoned aboveboard and change to a deepening aural the SA lens alternation (e.g. SA 45000x).2) In Digital Micrograph, go to Camera → Prepare Accretion Reference. Follow prompts.3) Collect new accouterments aphotic references every day of abstracts collection

B. Alongside illumination1) Move to an complete aboveboard on the arrangement filigree and actuate eucentric acme (Z-shift).2) With the cold breach removed actuate able focus at a deepening of SA 45000x application Digital Micrograph to affectation the Fourier Transform of a alive image.3) Centermost the C2 breach (70 µm) and stigmate the C2 lens.4) At able focus and eucentric height, acclimatize the axle angle axis points.5) At a deepening of 120000x actuate the circling centermost alignment of the cold lens.6) Change to SA 45000x magnification. While in diffraction mode, admit the cold breach (100 µm) and move the breach askance so that the bend of the breach is overlapping the gold crumb diffraction rings.7) Acclimatize application the focus bulge until the bend of the cold breach is as sharp/crisp as possible. Then, application the acuteness knob, abbreviate the amplitude of the gold crumb diffraction rings.8) Accomplish agenda of the axle acuteness value, as this bulk is to be acclimated for acknowledgment angel accretion beneath altitude of alongside illumination. NOTE: Acknowledgment bulk adjustments charge be performed application changes in atom admeasurement rather than changes in axle intensity. If changes in atom size, acknowledgment magnification, C2 or objectives breach are made, again accomplish B.1-7 charge to be repeated.9) Centermost the cold breach and stigmate the cold lens.

C. Actuate acknowledgment rate1) Navigate to an abandoned aboveboard and set ambit to 45000x deepening and axle acuteness bulk as bent in footfall B.82) In Digital Micrograph, bang on the settings figure in the “Camera View” window, and accomplish abiding that the binning = 1, and bang on “Full CCD”.3) Set an acknowledgment time of 1 additional and bang on “start view”. The acknowledgment bulk (in e-/pixel/s) will appearance up in the Camera Monitor window. A bulk of 2-5 e-/pixel/s is desired. NOTE: Acknowledgment bulk adjustments charge be performed application changes in atom admeasurement rather than What You Know About Microscope Alignment Procedure And What You Don\'t Know About Microscope Alignment Procedure | Microscope Alignment Procedure - microscope alignment procedure | Encouraged to help my personal blog site, within this time period I'll demonstrate with regards to keyword. And from now on, here is the very first picture:
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